Shiitake (Lentinula edodes) is a highly cultivated edible mushroom known to contain ergosterol, which can be converted into vitamin D₂ (ergocalciferol) through exposure to UV‑light wholesale shiitake mushroom extract fortified with vitamin D₂ is sourced from cultivated shiitake fruiting bodies that undergo controlled post‑harvest UV irradiation to elevate D₂ levels for use in skincare and nutraceutical formulations.
Manufacturing Process Of Shiitake Mushroom Extract From Vitamin D₂
1. Harvest & Cleaning – Fresh shiitake fruiting bodies are cleaned and optionally sliced or finely chopped to increase surface area (typically 0.5–3 mm slices or chopped pieces), which enhances UV conversion.
2. UV Irradiation – Samples are exposed to UV‐B (around 280–300 nm) and sometimes UV‑C wavelengths under controlled doses (e.g. 1.5–4 J/cm² for 100–180 min; UVC may follow) to convert ergosterol into vitamin D₂.
3. Drying & Crushing – After irradiation, the material is dried and ground to fine powder or oil‐extracted slurry.
4. Extraction (Powder or Oil)
• Powder form: ethanol or solvent extraction (e.g. reflux with ethanol, alkaline saponification, hexane partition, concentration and HPLC purification) to concentrate D₂
• Oil form: mixing with edible oil (sunflower, rapeseed, etc.), extraction under vacuum and nitrogen for many hours, then plate‑and‑frame filtration to produce vitamin D₂‑enriched mushroom oil
5. Standardization & QC – High‑performance liquid chromatography (HPLC) is used to quantify vitamin D₂, ensure ergosterol conversion, and certify purity and microbial/heavy‑metal safety.

Undersun product offers a natural, vegan‑friendly anti‑aging ingredient derived from shiitake mushrooms. The patented UV conversion and extraction protocols ensure high vitamin D₂ content and consistent quality.
Anti-Aging & Commercial Uses
Vitamin D₂is known for its antioxidant activity and support of skin barrier and repair processes, making it suitable for anti‑aging topical ingredients in skincare creams, serums, masks and supplements. Although vitamin D₃is more bio‑available in humans, D₂remains a widely accepted functional ingredient within vegan-certified, natural product lines. Commercial uses include:
|
Field / Use Case |
Extract Benefit |
Ideal Product Application |
|
Bone Health (Post‑menopause) |
Improves bone density and microarchitecture |
Women's health, bone support capsules |
|
Immune / Liver Support |
Anti-inflammatory, hepatoprotective synergy |
Immune‑support capsules or liquids |
|
Active Lifestyle Supplements |
Vegan vitamin D maintenance |
Sports nutrition, wellness powders |
|
Functional Food & Beverages |
Acceptable, convenient delivery vehicle |
Sachets, soups, drink mixes |
From Shiitake Mushroom Extract From Vitamin D₂For Customer Cases
1. Bone Health / Osteoporosis Support (Post-menopausal Women)
A pre‑clinical study using UV‑irradiated shiitake powder administered to ovariectomized rats showed improved femoral bone mineral density and trabecular architecture compared to controls on vitamin D-deficient diets. Vitamin D₂ was bioavailable, and bone structure markers were mitigated. This supports commercial use in women's supplements targeting bone strength and menopausal support.
2. Immune-Modulation & Liver Protection (Functional Wellness)
In a mouse model of immune-mediated hepatitis, vitamin D₂‑enriched shiitake extracts were shown to significantly reduce liver injury markers (ALT, AST), lower interferon‑γ levels, and improve histological outcomes versus non‑enriched mushroom extracts. A synergistic anti-inflammatory benefit was noted beyond vitamin D alone
A nutraceutical developer launching an immune‑support line included the extract as an adjuvant for liver-support formulations with humanized ingredients.
3. Vitamin D Maintenance in Active Individuals
A randomized trial involving recreationally active volunteers compared mushroom-derived D₂ capsules (1,000 IU/d) with D₃ capsules and placebo over 12 weeks. The D₂ group saw a 347 % rise in serum 25‑OH‑D₂ and maintained total vitamin D status similar to placebo, whereas D₃ markedly increased total 25‑OHD. While D₂ alone had modest effects, it was effective in maintaining baseline levels in vitamin‑D‑insufficient subjects.
A supplement brand including the extract in vegan sports multivitamins aimed at maintaining wellness during low-sun periods.

4. Consumer Dietary Trials (Human Supplementation Study in India)
In an open-label randomized trial with vitamin‑D–deficient participants aged 21–45, daily intake of shiitake‑based D₂ soup (prepared from UV-irradiated shiitake powder) for four weeks was compared to a commercial 50,000 IU D₂ capsule and placebo. The shiitake soup group experienced measurable rises in serum 25‑OHD over ~36 days, which tracked comparably to the high‑dose capsule group.
A illustrates consumer acceptance and efficacy in functional foods or sachet‑based supplements targeted at deficient populations.
Contact Us for Wholesale Orders
Whether you are sourcing ingredients for a new product line or scaling production, bulk ingredient suppliers is your reliable factory partner for Shiitake mushroom extract, Organic chaga powder, Cordyceps powder, Lion's mane powdr, Reishi powder, Turkey Tail & Tremella Extract secure your stock of 100kg / 275kg / 375kg / 1250kg / 2750kg / 4500kg, Undersun delivers consistent supply and value for dietary supplement brands! Contact us today for technical specifications, samples, and bulk pricing.
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