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Thin Layer Identification of Dandelion Extract
Jul 19, 2019

First,Dandelion Extract the identification conditions

Chromatography cylinder: specification 200×200

Developer: butyl acetate-methanol-water (14:5:5)

Inspection method: TLC


Second, the identification method

Take 5g of this product powder, add 30ml of methanol solution, heat and reflux for 30 minutes, filter, and evaporate the filtrate. The residue is dissolved in 10ml of water to be dissolved. It is extracted twice with 10ml of ethyl acetate, and the combined ethyl acetate solution is steamed. Dry, and the residue was dissolved in 1 ml of methanol to prepare a test solution.


Another dandelion reference medicine 5g was taken, and the same method was used to prepare a reference medicine solution. According to thin-layer chromatography (General Rule 0502), 10 μl of each of the above two solutions was aspirated and placed on the same silica gel G thin-layer plate, and the upper layer solution of butyl acetate-methanol-water (14:5:2) was used as a developing solvent. , unroll, remove, dry, and set under UV light (365nm). In the chromatogram of the test sample, fluorescent spots of the same color are displayed at positions corresponding to the chromatogram of the reference drug.


From left to right: 1. Sample 2. Reference herbs. Please feel free to contact us or send an email to [email protected]

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